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collagen 2  (SouthernBiotech)


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    Structured Review

    SouthernBiotech collagen 2
    Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) <t>collagen</t> <t>2</t> + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
    Collagen 2, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+type/Goat+Anti-Type+II+Collagen-UNLB/pmc12971017-76-5-12
    Average 93 stars, based on 30 article reviews
    collagen 2 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice"

    Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

    Journal: JBMR Plus

    doi: 10.1093/jbmrpl/ziag021

    Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.
    Figure Legend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

    Techniques Used: Immunohistochemical staining, Staining

    Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.
    Figure Legend Snippet: Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

    Techniques Used: Immunohistochemical staining, Staining

    Related Articles

    Staining:

    Article Title: Kidney disease reprograms microbiome-host signaling to promote heart failure
    Article Snippet: For wheat germ agglutinin (WGA) staining, cryosections were incubated with WGA Fluorescein (1:800, Vector Laboratories) and DAPI staining solution (1:1000, Biotium) in TBST + 5% NDS for 2 h at RT. .. For extracellular matrix (ECM) protein staining, cryosections were incubated with primary antibodies, rabbit anti-fibronectin (1:100, polyclonal, abcam) and goat anti-type I collagen (1:50, polyclonal, Southern Biotech), in TBST + 5% NDS overnight at 4 °C. .. After washing in TBST, cryosections were incubated with secondary antibodies, donkey anti-rabbit IgG Alexa Fluor Plus 488 (1:1000, Thermo Fisher) and anti-goat IgG Alexa Fluor Plus 555 (1:1000, Thermo Fisher), together with DAPI (1:1000, Biotium) in TBST + 5% NDS for 1 h at RT.

    Article Title: Bone Marrow Aspirate Concentrate Combined with Ultra-Purified Alginate Bioresorbable Gel Enhances Intervertebral Disc Repair in a Canine Model: A Preclinical Proof-of-Concept Study
    Article Snippet: After protein blocking using Protein Block Serum-Free (Dako, Agilent Technologies, Cat # X0909, Santa Clara, CA, USA), the sections were treated with 3% H O . .. Thereafter, goat anti-type I collagen (Southern Biotech, Cat# 1310-01, Birmingham, AL, USA) and anti-type II collagen mouse (Kyowa Pharma Chemical Co., Ltd., Cat# F-57, Toyama, Japan) were used as primary antibodies, while histfine simple stain max-PO(G) (Nichirei Biosciences, Cat# 414162, Tokyo, Japan) and envision + system-HRP labelled polymer anti-mouse (Dako, Cat# 4001) were used as secondary antibodies for type I and type II collagen, respectively. .. Finally, the sections were stained with DAB (Dako, Cat# K3468) and hematoxylin, and washed with water.

    Incubation:

    Article Title: Kidney disease reprograms microbiome-host signaling to promote heart failure
    Article Snippet: For wheat germ agglutinin (WGA) staining, cryosections were incubated with WGA Fluorescein (1:800, Vector Laboratories) and DAPI staining solution (1:1000, Biotium) in TBST + 5% NDS for 2 h at RT. .. For extracellular matrix (ECM) protein staining, cryosections were incubated with primary antibodies, rabbit anti-fibronectin (1:100, polyclonal, abcam) and goat anti-type I collagen (1:50, polyclonal, Southern Biotech), in TBST + 5% NDS overnight at 4 °C. .. After washing in TBST, cryosections were incubated with secondary antibodies, donkey anti-rabbit IgG Alexa Fluor Plus 488 (1:1000, Thermo Fisher) and anti-goat IgG Alexa Fluor Plus 555 (1:1000, Thermo Fisher), together with DAPI (1:1000, Biotium) in TBST + 5% NDS for 1 h at RT.

    Article Title: Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model
    Article Snippet: .. After blocking with Blocking One Histo (06349-64; Nacalai Tesque Inc., Japan) in 1× PBST (1× PBS, 0.1% Tween 20) at room temperature for one hour, the sections were incubated at 4°C overnight with the following primary antibodies: goat anti-type I collagen (1:200; 1310-01; SouthernBiotech, USA), rat anti-F4/80 (1:200; ab6640; Abcam, UK), and rabbit anti-laminin (1:200; ab11575; Abcam). .. For anti-type I collagen, the immune complexes were detected using anti-goat IgG H&L (HRP) (1:500; ab97110; Abcam) and ImmPACT DAB (SK-4105; Vector Laboratories, USA).

    Article Title: Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model
    Article Snippet: .. After blocking with Blocking One Histo (06349-64; Nacalai Tesque Inc., Japan) in 1× PBST (1× PBS, 0.1% Tween 20) at room temperature for one hour, the sections were incubated at 4°C overnight with the following primary antibodies: goat anti-type I collagen (1:200; 1310-01; SouthernBiotech, USA), rat anti-F4/80 (1:200; ab6640; Abcam, UK), and rabbit anti-laminin (1:200; ab11575; Abcam). .. For anti-type I collagen, the immune complexes were detected using anti-goat IgG H&L (HRP) (1:500; ab97110; Abcam) and ImmPACT DAB (SK-4105; Vector Laboratories, USA).

    Polymer:

    Article Title: Bone Marrow Aspirate Concentrate Combined with Ultra-Purified Alginate Bioresorbable Gel Enhances Intervertebral Disc Repair in a Canine Model: A Preclinical Proof-of-Concept Study
    Article Snippet: After protein blocking using Protein Block Serum-Free (Dako, Agilent Technologies, Cat # X0909, Santa Clara, CA, USA), the sections were treated with 3% H O . .. Thereafter, goat anti-type I collagen (Southern Biotech, Cat# 1310-01, Birmingham, AL, USA) and anti-type II collagen mouse (Kyowa Pharma Chemical Co., Ltd., Cat# F-57, Toyama, Japan) were used as primary antibodies, while histfine simple stain max-PO(G) (Nichirei Biosciences, Cat# 414162, Tokyo, Japan) and envision + system-HRP labelled polymer anti-mouse (Dako, Cat# 4001) were used as secondary antibodies for type I and type II collagen, respectively. .. Finally, the sections were stained with DAB (Dako, Cat# K3468) and hematoxylin, and washed with water.

    Blocking Assay:

    Article Title: Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model
    Article Snippet: .. After blocking with Blocking One Histo (06349-64; Nacalai Tesque Inc., Japan) in 1× PBST (1× PBS, 0.1% Tween 20) at room temperature for one hour, the sections were incubated at 4°C overnight with the following primary antibodies: goat anti-type I collagen (1:200; 1310-01; SouthernBiotech, USA), rat anti-F4/80 (1:200; ab6640; Abcam, UK), and rabbit anti-laminin (1:200; ab11575; Abcam). .. For anti-type I collagen, the immune complexes were detected using anti-goat IgG H&L (HRP) (1:500; ab97110; Abcam) and ImmPACT DAB (SK-4105; Vector Laboratories, USA).

    Article Title: Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model
    Article Snippet: .. After blocking with Blocking One Histo (06349-64; Nacalai Tesque Inc., Japan) in 1× PBST (1× PBS, 0.1% Tween 20) at room temperature for one hour, the sections were incubated at 4°C overnight with the following primary antibodies: goat anti-type I collagen (1:200; 1310-01; SouthernBiotech, USA), rat anti-F4/80 (1:200; ab6640; Abcam, UK), and rabbit anti-laminin (1:200; ab11575; Abcam). .. For anti-type I collagen, the immune complexes were detected using anti-goat IgG H&L (HRP) (1:500; ab97110; Abcam) and ImmPACT DAB (SK-4105; Vector Laboratories, USA).



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    Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

    Journal: JBMR Plus

    Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

    doi: 10.1093/jbmrpl/ziag021

    Figure Lengend Snippet: Long bone growth of 12-wk-old male Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05, ** p < .01, **** p < .0001.

    Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.

    Techniques: Immunohistochemical staining, Staining

    Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

    Journal: JBMR Plus

    Article Title: β 2 -adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in mice

    doi: 10.1093/jbmrpl/ziag021

    Figure Lengend Snippet: Long bone growth of 12-wk-old female Adrb2 flox/flox and Adrb2 Col2a1-Cre mice. (A) Femur and (B) tibia length. (C) Femoral growth plate thickness. Thickness of the (D) non-hypertrophic and (E) hypertrophic zone of the femoral growth plate. (F) Collagen 10 + chondrocytes, (G) collagen 2 + chondrocytes, and (H) runt-related transcription factor 2 (Runx2) + chondrocytes in the femoral growth plate. (I) Histological (toluidine blue) and immunohistochemical (collagen 10, collagen 2, Runx2) staining of the femoral growth plate. Scale bar = 50 μ m . n = 4-6. * p < .05.

    Article Snippet: For immunofluorescence double staining of Collagen 2 (goat anti-mouse Col2, 1:50, #1320-01, Southern Biotech) and β 2 -AR (rabbit anti-mouse β 2 -AR, 1:50, #PA5-77283, Thermo Fisher Scientific), decalcified sections of intact and fractured femora were used.

    Techniques: Immunohistochemical staining, Staining